Newcastle Disease and Infectious Bronchitis Disease Vaccination Programs in Field Study

This experiment was conducted by using (32000) chicks classified into three houses (18000 chicks in first house, 5000 chicks in second house and 9000 chicks in third house). The first group was inoculated with inactivated oil emulsion vaccine contain NDV (La Sota) and AIV (H9N2) subcutaneous at one day, in the same time vaccinated with mixed vaccine of NDV (La Sota) and IBV H120 by spraying route followed by booster dose by the same vaccine (NDV (La Sota) and IBV H120) at day 5 via drinking water and at the eighteen day vaccinated with IBV H120 via drinking water, while the second group vaccinated with mixed vaccine (NDV (La Sota) and IBV H120) at one day by spray route, at ten day vaccinated with inactivated oil emulsion vaccine contain NDV (La Sota) and AIV (H9N2) injection subcutaneous and the same time vaccinated with NDV (La Sota) by spray route, where as the third group was vaccinated with inactivated oil emulsion vaccine contain NDV (La Sota) and AIV (H9N2) injection subcutaneous at fifth day in the same time vaccinated with mixed vaccine from NDV (La Sota) and IBV H120 by droplet route in eyes and at the eighteen day vaccinated with IBV H52 via drinking water. ELISA test and Histopatholoical examination were used to evaluated the immune response against NDV and IBV, the results revealed high antibody (Ab) titres against IBV in first group that received more one mixed vaccine but give pathological changes in trachea and kidney as compared with other group that give low titre with least histological changes.